Plasmid

Part:BBa_K5048054:Design

Designed by: Yiheng Liao   Group: iGEM24_WHU-China   (2024-09-22)


plasmid pBAD luxR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1859
    Illegal PstI site found at 1839
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1859
    Illegal NheI site found at 897
    Illegal NheI site found at 920
    Illegal PstI site found at 1839
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1859
    Illegal BglII site found at 1835
    Illegal BamHI site found at 1825
    Illegal XhoI site found at 1831
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1859
    Illegal PstI site found at 1839
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1859
    Illegal PstI site found at 1839
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 216
    Illegal BsaI site found at 2038
    Illegal BsaI site found at 3105
    Illegal SapI.rc site found at 4187


Design Notes

We replace the T7 promoter with araBAD promoter to prevent the leakage of promoter.


Source

complete synthesis

References